Table 2

Multivariate analysis of gene expression in normal-appearing colon mucosa of 23-week-old APCmin mice compared with colon mucosa of wild-type mice

Colons were removed from animals, and any polyps were removed. The colons were then divided into six segments, the colon mucosa was isolated, and gene expression was determined as described in the “Materials and Methods,” with values for APCmin mice compared with those for wild-type mice. Multivariate analysis was performed on these values as described in the “Materials and Methods,” in which the significance of the difference in expression between APCmin and wild-type mice was determined for each gene in the presence of all of the other genes. For this analysis, six mice were used for each group (APCmin and wild-type), and one mucosa sample was analyzed per segment per mouse.

GeneColon segmenta
123456
Cox-2b+++++++++++++++
CXCR2++++++++++
MIP-2++++++++++
Gro-α+++++
OPN
  • a −, P > 0.05; +, P < 0.05; ++, P < 0.01; +++, P < 0.001; ++++, P < 0.0001.

  • b Cox-2, cyclooxygenase-2; CXCR2, CXC cytokine receptor-2; MIP-2, macrophage inflammatory protein-2; Gro-α, growth-related oncogene-α; OPN, osteopontin.