Table 3

Gene expression levels in morphologically normal colon mucosa from human colon cancer patients as compared with noncancer subjects

For Table 3A, colon mucosa samples were isolated from the sigmoidal-rectal region of noncancer subjects (78 samples from 12 individuals) and from the adjacent normal-appearing mucosa of patients with sigmoidal-rectal cancer (62 samples from 5 patients). For Table 3B, the colon mucosa samples were isolated from the ascending region of noncancer subjects (39 samples from 11 individuals) and from the adjacent normal-appearing mucosa of patients with ascending colon cancer (65 samples from 4 patients). Samples were analyzed for gene expression as described in the “Materials and Methods.” Means ± SD are given for noncancer subjects, and ranges are given for cancer patients. Multivariate analysis was then performed on each gene taken in relation to all of the other genes to determine the significance of the difference between individuals with or without cancer.

A. Sigmoidal-rectal colon
No.GeneNormal subjects (mean ± SD)Cancer patients (range)P
1CXCR2a1.30 ± 1.110.81–210<0.01
2Gro-α2.93 ± 6.930.78–105NSb
3IL-82.25 ± 2.631.22–820.0001
4COX-21.80 ± 2.630.91–660.001
5OPN1.55 ± 2.040.94–580.0001
6Gro-γ1.92 ± 3.340.80–37NS
7MCSF-11.54 ± 1.401.54–310.0001
8COX-11.22 ± 0.870.12–9.58NS
9CD441.12 ± 0.560.54–6.52<0.05
10c-myc1.24 ± 0.820.12–4.76NS
11Cyclin D1.28 ± 0.840.43–4.44NS
12PPAR-α1.10 ± 0.620.02–2.87NS
13PPAR-δ1.15 ± 0.550.02–1.90<0.01
14p211.04 ± 0.290.40–1.68<0.01
15PPAR-γ1.07 ± 0.400.01–1.28<0.01
B. Ascending colon
1CXCR21.32 ± 1.081.90–90<0.05
2Gro-α1.60 ± 2.080.46–30NS
3IL-81.66 ± 1.621.32–183<0.05
4COX-21.84 ± 3.042.96–1530.0001
5OPN1.53 ± 1.319.24–1530.0001
6Gro-γ1.40 ± 1.410.63–11NS
7MCSF-11.68 ± 1.624.01–400.0001
8COX-11.17 ± 0.750.84–45<0.001
9CD441.11 ± 0.510.99–140.0001
10c-myc1.16 ± 0.630.39–10.82NS
11Cyclin D1.38 ± 1.080.12–13.15NS
12PPAR-α1.16 ± 0.580.22–4.09NS
13PPAR-δ1.13 ± 0.550.02–7.08<0.05
14p211.09 ± 0.400.04–2.66NS
15PPAR-γ1.08 ± 0.420.01–1.140.01
  • a CXCR2, CXC cytokine receptor 2; Gro-α and -γ, growth-related oncogene-α and -γ; IL-8, interleukin-8; COX-1 and −2, cyclooxygenase-1 and −2; OPN, osteopontin; MCSF-1, macrophage-colony-stimulating factor; PPAR-α, -δ, and -γ, proliferating peroxisome-activating receptor-α, -δ, and -γ.

  • b NS, not significant at P < 0.05 level.