Table 1

Experimental design for bioassay with PBITa

GroupTreatmentNo. of ratsAmount administered (ml)Dose admin. (mg/kg body weight)Diet
1DMSO + H2Ob250.20Control AIN-76A
2None2500AIN-76A + PBIT (50 ppm)
3None2500AIN-76A + PBIT (100 ppm)
4NMBAc500.20.25Control AIN-76A
5NMBA500.20.25AIN-76A + PBIT (50 ppm)
6NMBA500.20.25AIN-76A + PBIT (100 ppm)
  • a PBIT, S,S′-1,4-phenylene-bis(1,2-ethanediyl)bis-isothiourea; NMBA, N-nitrosomethylbenzylamine.

  • b DMSO + H2O, vehicle for NMBA.

  • c 0.25 mg/kg body weight NMBA injected s.c. three times per week for 5 weeks.