Table 3

Effect of HKI-272 on cell proliferationa

Cell lineHER-2Epidermal growth factor receptorIC50 (nm)b
3T3700 ± 78 (n = 5)
3T3/neu+++3 ± 0.14 (n = 5)
SK-Br-3++++2 ± 0.18 (n = 4)
BT 474++++2 ± 0.06 (n = 4)
A431++++81 ± 9 (n = 5)
MDA-MB-435960 ± 165 (n = 2)
SW620690 ± 84 (n = 5)
  • a Cells were incubated with various concentrations of compound for 2 days (6 days for BT474). Cell survival was determined using a protein binding dye assay. The concentration of drug that inhibits cell proliferation by 50% is shown. Relative expression of and epidermal growth factor receptor and HER-2 in the cell lines are indicated.

  • b Mean and SE are shown with the number of independent determinations in parentheses.