Table 5

Effect of HKI-272 on cell cycle progressiona

HKI-272 (nm)Sub-G1G0-G1SS (BrdUrd)∗G2-M
0861212611
0.2963161813
0.41165121413
1769101214
21166121011
  • a BT474 cells were treated with the indicated concentrations of HKI-272 for 12–16 h at 37°C. Untreated and treated cells were pulse labeled for 30 min with bromodeoxyuridine (BrdUrd). Cells were stained with BrdUrd antibodies/FITC conjugates, counterstained with propidium iodide, and analyzed by flow cytometry. The percentage of cells in sub-G1, G1, S, and G2 phases of the cell cycle are shown, as determined from the histogram of propidium iodide-stained cells. Additionally, the percentage of cells in S phase was confirmed by BrdUrd uptake (∗). This data was use to calculate the IC50 shown in Table 4 <$REFLINK> .