Table 2

Cell cycle percentages for SKBR3 parental and trastuzumab-resistant cells

Untreated SKBR3 parental cells, parental cells treated with 4 μg/ml Trasa for 72 h, and Tras-resistant pools 1 and 2 maintained at 4 μg/ml Tras were fixed, stained with propidium iodide, and analyzed for DNA content by flow cytometry. The percentage of cells in each cell cycle phase is shown.

CellSub-G1G1SG2-M
Parental5.48 ± 0.1658.84 ± 3.2210.72 ± 1.0825.64 ± 0.49
Parental + Tras5.14 ± 0.0463.9 ± 0.026.99 ± 1.2525.04 ± 1.27
 Pool 18.34 ± 0.5159.87 ± 0.0933.69 ± 1.636.44 ± 1.55
 Pool 27.48 ± 0.7859.05 ± 3.2629.33 ± 1.3111.62 ± 1.95
  • a Tras, trastuzumab.