Table 4

Comparison between Q-MSPa and QM-MSP

The percentage of methylation (% M) was calculated as: (the number of copies of methylated DNA divided by the number of copies of unmethylated + methylated DNA) × 100, using absolute quantitation. The positive control for unmethylated DNA was HSD, and for methylated DNA was 231.

% M
Q-MSPQM-MSP
RASSF1A
 19599
 28081
 33941
 41221
 52834
 HSD00
 231100100
 Water00
TWIST
 19670
 200
 35426
 413
 505
 HSD00
 231100100
 Water00
HIN1
 19582
 28174
 38248
 44116
 54743
 HSD00
 231100100
 Water00
Cyclin D2
 14725
 200
 310
 41922
 520
 HSD00
 231100100
 Water00
  • a Q-MSP, quantitative methylation-specific PCR; QM-MSP, quantitative multiplexed methylation-specific PCR; HSD, human sperm DNA.