Table 4

Association of CpG island hypermethylation in 5′ gene regions with satellite DNA hypomethylationa

A. Association of satellite DNA hypomethylation and gene hypermethylation in ovarian cancers
Chr1 Sat2Chr1 SatαAll centromeres
GenesAssocn.bPGenesAssocn.PGenesAssocn.P
RNR1 <0.001 CDH13 <0.001 CDH13 0.014
CDH13 0.001 BLT10.03CALCA+0.13
CALCA+ 0.003 RNR10.09RNR10.20
ESR10.11MCJ0.09MCJ0.36
APC+0.24RASSF1A+0.11SOCS1+0.43
BLT1+0.42ESR1+0.14CDH1+0.51
CDH1+0.53CDH1+0.31APC+0.52
PGR0.55SOCS10.49TERT+0.54
SOCS10.60PTGS20.55BLT1+0.55
PTGS2+0.60TERT0.55DR30.71
MCJ+0.70APC0.61PTGS2+0.71
TNFRSF120.78PGR0.68IGSF4+0.72
RASSF1A0.91IGSF40.73PGR0.86
TERT0.92DR3+0.79RASSF1A0.90
IGSF4+0.94CALCA+0.83ESR1+1.0
B. Assocn. of satellite hypomethylation with gene hypermethylation in breast cancers
GenesChr1 Sat2Chr1 SatαAll centromeres
Assocn.PAssocn.PAssocn.P
CDH13 0.030 0.10 0.030
RNR10.150.12 0.030
CALCA0.490.840.055
  • a By the Mann-Whitney U test, the association of 15 gene hypermethylation markers in ovarian cancer specimens are ranked according to the strength of their association with three different categories of satellite DNA hypomethylation (Sat2, juxtacentromeric Sat2 in Chr1; Chr1 Satα, centromeric Satα in Chr1; All centromeres, all centromeric Satα DNA). The satellite DNA hypomethylation scores were categorized as to those ≤2 versus those >2. The PMR values of the 15 genes were used as continuous variables. Significant associations after adjustment for multiple comparisons are indicated in bold type.

  • b Assocn. +, a positive relationship (not necessary significant) between the extent of satellite DNA hypomethylation and DNA hypermethylation; Assocn. −, tumors with hypermethylation of this gene marker had a lower frequency of satellite DNA hypomethylation than seen for the complete collection of tumors.