Table 1

Binding affinity and activity in the Matrigel plug assay of multimers of HHPHG

SequenceTropomyosin bindingMatrigel plug
IC50m)SD% inhibition
HHPHG922.868*
(HHPHG)211.62.832
Ac-(HHPHG)2-NH226.92.541
(HHPHG)31.250.3141
Ac-(HHPHG)3-NH21.560.5353
(HHPHG)40.2560.0769
Ac-(HHPHG)4-NH20.2450.1284
• NOTE. The peptides were tested in the tropomyosin binding assay as described in Fig. 1 <$REFLINK> . The data are the mean and SD of n = 3 experiments. The peptides were also tested in the Matrigel plug assay as described in Fig. 4A <$REFLINK> . The data shown are from a representative experiment. HHPHG was tested at a concentration of 300 μm and the other peptides at 0.5 μm. The relationship between the number of repeats and the IC50 is exponential as determined by nonlinear regression analysis and is defined as IC50 = 732.7×e(-2.075×n), where n is the number of HHPHG repeats.

• * Tested at 300 μm; all other peptides at 0.5 μm.