Sequence | Tropomyosin binding | Matrigel plug | |
---|---|---|---|
IC_{50} (μm) | SD | % inhibition | |
HHPHG | 92 | 2.8 | 68^{*} |
(HHPHG)_{2} | 11.6 | 2.8 | 32 |
Ac-(HHPHG)_{2}-NH_{2} | 26.9 | 2.5 | 41 |
(HHPHG)_{3} | 1.25 | 0.31 | 41 |
Ac-(HHPHG)_{3}-NH_{2} | 1.56 | 0.53 | 53 |
(HHPHG)_{4} | 0.256 | 0.07 | 69 |
Ac-(HHPHG)_{4}-NH_{2} | 0.245 | 0.12 | 84 |
NOTE. The peptides were tested in the tropomyosin binding assay as described in Fig. 1 <$REFLINK> . The data are the mean and SD of n = 3 experiments. The peptides were also tested in the Matrigel plug assay as described in Fig. 4A <$REFLINK> . The data shown are from a representative experiment. HHPHG was tested at a concentration of 300 μm and the other peptides at 0.5 μm. The relationship between the number of repeats and the IC_{50} is exponential as determined by nonlinear regression analysis and is defined as IC_{50} = 732.7×e^{(-2.075×n)}, where n is the number of HHPHG repeats.
* Tested at 300 μm; all other peptides at 0.5 μm.