Table 1

Arginase I produced by TAMC inhibits OVA-specific OT-1 responses in vitro

No coculturesTAMCNon-TAMCTAMC + Nor-NOHATAMC + l-Arg
Nonstimulated 33 * 48.5 33 88.5 42
(41)(23)(36)(40)(32)
OVA257–264 20713 217 23842 20517 24562
(1050)(47.3)(5476)(2849)(3023)
CD3 + CD28 26062 507 28996 27116 28562
(3535)(78)(4462)(1254)(4082)
  • NOTE. OT-1 were stimulated with 1 μg/ml OVA257–264 (SIINFEKL) peptide and cocultured with TAMC, non-TAMC, or TAMC plus Nor-NOHA or exogenous l-Arg. Proliferation was tested after 72 h of culture.

  • * Mean of three experiments (±SD).