Table 2

Arginase I produced by TAMC inhibits OVA-specific OT-2 responses in vitro

No coculturesTAMCNon-TAMCTAMC + Nor-NOHATAMC + l-Arg
Nonstimulated 19.5 * 22 61 33 23
(37)(39)(41)(29)(45)
OVA323–339 17073 164 15159 15562 19562
(2050)(67.8)(1023)(652)(2156)
CD3 + CD28 19209 356 18572 23267 22341
(1858)(64)(1429)(2467)(3602)
  • NOTE. OT-2 splenocytes were stimulated with 3 μg/ml OVA323–339 (ISQAVHAAHAEINEAGR) and co-cultured with TAMC, non-TAMC or TAMC plus Nor-NOHA or exogenous l-Arg. Proliferation was tested after 72 h of culture.

  • * Mean of three experiments (±SD).