Table 1

Primer sequence

No.TypePrimer sequenceCodon, region in KIT
1GenomicF: 5′-CCCAAGTGTTTTATGTATTT449–514, exon 9
R: 5′-ATGGTGTGATGCATGTATTA
2GenomicF: 5′-TCCAGAGTGCTCTAATGAC550–591, exon 11
R: 5′-AGGTGGAACAAAACAAAGG
3GenomicF: 5′-TACTGCATGCGCTTGACATC627–664, exon 13
R: 5′-CCAAGCAGTTTATAATCTAGC
4GenomicF: 5′-TTCTACATGTCCCACTTGATT715–744, exon 15
R: 5′-AGCATGATATACATACTCTCTG
5GenomicF: 5′-GTGAACATCATTCAAGGCGT788–828, exon 17
R: 5′-CCTTTGCAGGACTGTCAAGCA
6cDNAF: 1265: 5′-TCCTGACTTACGACAGGCTCGT409–524, extracellular + TM
R: 1611: 5′-ACATCATGCCAGCTACGATT
7cDNAF: 1577: 5′-ACACCCTGTTCACTCCTTTGCTGA519–706, TM + kinase I
R: 2136: 5′-GACTCCTTTGAATGCAGAAGA
8cDNAF: 2071: 5′-CGTGATTCATTTATTTGTTC684–830, kinase insert + kinase 2
R: 2510: 5′-CATCCACTTCACAGGTAGTC
9cDNAF: 2387: 5′-TTCACAGAGACTTGGCAGCCAG789–976, kinase 2 + COOH-terminal
R: 2961: 5′-TCCTGGAGGGGTGACCCAAACACT
  • Abbreviations: F, forward; R, reverse; TM, transmembrane.