Table 1

Percentage of aneuploid cells and centrosome abnormalities

A. Karyotype (p14–p18) aneuploid cells (%)B. FISH aneuploid cells (%)C. Centrosome abnormal centrosomes (%)
UnculturedCulturedUncultured
Me-Ec75 (p14)34 §85 (p6)32
Li-EC80 (p14)16 87 (p6)26
Sk-EC15 (p15)3 8 (p19)4
Ad-EC0 (p18)0ND ∗∗1
  • Abbreviations: EC, endothelial cell; ME, melanoma; Li, liposarcoma; Sk, skin; Ad, adipose; p, passage number.

  • Karyotype of cultured EC. Percent of aneuploid cells; 20 cells are scored.

  • FISH analysis of uncultured (left) and cultured (right) EC. Percent of aneuploid cells; 100 cells were scored.

  • § Me-EC: 15% had 3, 18% had 4, and 1% had 6 copies of chromosome 17.

  • Li-EC: 12% had 3 and 4% had 4 copies of chromosome 17.

  • Sk-EC: 3% had 3 copies of chromosome 17.

  • ∗∗ No data available for cultured Ad-EC, but karyotype at p18 shows 0% aneuploidy.

  • Percent of cells with abnormal centrosomes; 100 uncultured cells were scored.