Functional measurements | Hela (304I) | |
---|---|---|
Glucose | Galactose | |
Doubling time^{a} | 18.4 ± 01 | 58 ± 02 |
Respiratory rate^{b} | 1.35 ± 0.23 | 2.72 ± 0.46 |
Cytochrome c oxydase activity^{c} | 5.6 ± 1.38 | 17.35 ± 5.04 |
Matrix pH^{d} | 8.03 ± 0.05 | 7.65 ± 0.10 |
Matrix pH after CCCP^{e} | 6.9 ± 0.12 | 6.82 ± 0.19 |
Matrix redox state^{f} | 93 ± 3.0 | 74 ± 2.5 |
^{a} The doubling time (DT) was calculated according to the formula: DT = 0.693.t/1n (N/N_{0}) with t (elapsed time), N_{0} (starting number of cells), N (final number of cells). DT is expressed in hours (n = 2 different experiments).
^{b} Expressed in nmol O_{2}/min/10^{6} cells (n ≥ 4).
^{c} Expressed in nmol O_{2}/min/10^{6} cells (n ≥ 4).
^{d} n ≥ 10.
^{e} n ≥ 10.
^{f} Expressed as percentage reduction of rosGFP1 (n ≥ 3).