Table 1

Live cell functional measurements demonstrate a more active state of oxidative phosphorylation in cells grown in galactose medium compared with glucose

Functional measurementsHela (304I)
GlucoseGalactose
Doubling timea18.4 ± 0158 ± 02
Respiratory rateb1.35 ± 0.232.72 ± 0.46
Cytochrome c oxydase activityc5.6 ± 1.3817.35 ± 5.04
Matrix pHd8.03 ± 0.057.65 ± 0.10
Matrix pH after CCCPe6.9 ± 0.126.82 ± 0.19
Matrix redox statef93 ± 3.074 ± 2.5
  • a The doubling time (DT) was calculated according to the formula: DT = 0.693.t/1n (N/N0) with t (elapsed time), N0 (starting number of cells), N (final number of cells). DT is expressed in hours (n = 2 different experiments).

  • b Expressed in nmol O2/min/106 cells (n ≥ 4).

  • c Expressed in nmol O2/min/106 cells (n ≥ 4).

  • d n ≥ 10.

  • e n ≥ 10.

  • f Expressed as percentage reduction of rosGFP1 (n ≥ 3).