Table 2

Substrate-dependent changes in mitochondrial morphology

Morphometric measurementsHela (304I)
GlucoseGalactose
Mitochondria/Cella5.4 ± 0.944.2 ± 1.96
Mitochondrial profiles/Cellb15.6 ± 6.819.8 ± 7.3
Mitochondrial section areac0.31 ± 0.220.22 ± 0.18
Mitochondrial tubular diameterd0.48 ± 0.080.32 ± 0.07
Intracristal/mitochondrial areae8 ± 124 ± 3
Matrix densityf11.6 ± 345.6 ± 8
Cristal/inner membraneg1.5 ± 0.663.6 ± 1.6
Number of inclusion bodiesh125
  • a Total mitochondrial area expressed as percentage of total cell area transmission electron microscopy (TEM) n ≥ 100.

  • b Average number of mitochondrial profiles within cell section (TEM) n ≥ 100.

  • c Average area of a mitochondrial cross-section (TEM) n ≥ 100.

  • d Average diameter of the mitochondrial tubule (fluorescence), n ≥ 100.

  • e Total cristal area expressed as percentage of total mitochondrial area, n ≥ 100.

  • f Average density of matrix (TEM) n ≥ 100.

  • g Total cristal membrane divided by inner membrane n ≥ 100.

  • h Number of inclusion bodies observed n ≥ 100.