Table 1

Fluorescent, bioluminescent and PETa reporter gene expressions exhibited by different triple fusion constructs in comparison to the respective positive controls assayed from transiently transfected 293T cells (tTK and hRL activities are normalized with cotransfected β-Gal activity)

Constructs% TK activity% wTK activity% hRL activity% FL activityRFP/eGFP/mRFP (fluorescence activity by microscopy)
hrl-rfp-ttk39.727.8Medium
fl-rfp-ttk29.622.1Low
hrl-egfp-ttk5033High
fl-egfp-ttk4320High
hrl-mrfp-ttk14954High
fl-mrfp-ttk10053.6Medium
hrl-rfp-wttk7644.7Medium
fl-rfp-wttk61.662.6Low
ttk100
wttk100
hrl100
fl100
mrfplVery high
  • a PET, positron emission tomography; eGFP, enhanced green fluorescence protein; β-Gal, β-galactosidase.