Table 3

Summary of mutations, allelic losses, and the relationship to protein expression in sporadic colorectal cancer with the methylation status of the PTEN promoter

Case no.PTEN methylationMSIa statusMutations at (A)6LOH at 10q23PTEN expressionb
Exon 7Exon 8D10S215D10S541D10S1765
UC15+MSI-LwtwtnsLOHLOH
UC29+MSI-Hwtwtnsnsns++
UC51+MSI-HwtMutnsnsns+
UC75+MSI-HMutwtnsnsns
CAL31+MSI-HMutwtninsni+
CAL42+MSI-Hwtwtnsnsns+
CAL65+MSI-Hwtwtnsnsns++
CAL79+MSI-Hwtwtnsnsns+
CAL88+MSI-LwtwtnsnsLOH+
CAL97+MSI-HMutwtninsns
UC9MSI-Hwtwtnsnsns++
UC10MSI-Hwtwtnsnsns++
UC16MSI-LwtwtLOHnsns+
UC20MSI-Lwtwtnsnsns++
UC26MSI-Hwtwtninsns++
UC45MSI-LMutwtnsnsns++
UC61MSSwtwtnsnsLOH++
UC62MSSwtwtnsnsns++
CAL7MSI-Hwtwtnsnsni++
CAL8MSI-HwtMutninsns+
CAL16MSI-Hwtwtnsnsns++
CAL33MSI-LwtwtnsnsLOH++
CAL46MSI-LwtwtnsLOHLOH+
CAL51MSI-LwtMutnsnsns++
CAL103MSI-Hwtwtnsnsns++
CAL163MSI-Hwtwtnsnsns++
  • a MSI, microsatellite instability; LOH, loss of heterozygosity; MSI-L, MSI-low; wt, wild-type allele; ns, no significant loss; MSI-H, MSI-high; Mut, mutated; ni, not informative.

  • b PTEN protein expression: normal staining, ++; weak staining, +; absence of staining, −.