Table 1

RT-PCRa analysis

BAC clones, EST and STS Marker refer to the information obtained from Ensembl genome data resource. All BAC clones are included in RPCI-11 human male BAC library.

BACEST/geneSTS MarkerForward primerReverse primerFBRec1Karpas 1718OCI-Ly4
360A9AW664738D13S14575′-gccacatggtcaggttaaac5′-cttcactaccttgcgactctNDND
27D9AA3091625′-ggctctcatttagctaaatg5′-aagaagtggttgagacaaca+b+++
15N8No EST
321E13AW236754D13S11755′-gagatggcacagcagttgaa5′-tagttcaaactctacctgcaNDND
447M23No EST
370B1LOC1217235′-aactactggtgaggactgca5′-caagttccccttctgcagaaNDND
AW0598675′-aaggcttagctattatgctg5′-acaatgaggaaaatctccca++c++++++
275J18BC042969D13S18185′-ctggtcacacatccacaatg5′-cagtggaatctgagtcctag++++++
AA6282995′-cagaaggagtgttaagtctg5′-caagaagaagctgccagtat++++++++
143O10No EST
309H8BG183515D13S12395′-ctcatgactgtaatcccagc5′-gtgatgccgtgaaatgagtc++++
BG1919815′-ttcagtgacctcactgactg5′-gaggattttgcagtcatcgcNDND
114G1LOC160824D13S7675′-aggttttgtcagccacacct5′-gaactatccgtaccttgtcc++++
75N6AA3982285′-ctgtaccattgtgcccagaa5′-atgactcagtccttctggctNDND
86C3No ESTD13S265
51A2No EST
18M3No EST
388D4LOC1447745′-cactgtggcagttatacggt5′-caatggttttccgcaccagtNDND
LOC1217275′-cctgggaaggatggtttctt5′-ttacaacacaaggggcacac+++++
409J23No EST
392A19LOC1217295′-ggagccattacttcaagacc5′-agaccagtacttgtccagct+++++++
BG7761865′-tggacacgtgagtgtgtttc5′-atgagctcaggcagctctat++++
LOC1217285′-tgataggagacagacctgac5′-tccagtcatcctgaggtagaNDND
BG1860785′-tcacacagtgtgactcacag5′-tgatctcctctctgtagtgcND+
AA4878825′-acccaagggaatattgacac5′-gaagctagggaagcagtattND+
BM5429915′-tgcagtaggtggcaatctca5′-tcgacttggactccatgagaNDND
505P2BM695971D13S12345′-ccttggagtgcttaaggtag5′-actagggctctttgtagaccND++
158A8AI0272785′-gctgtgattgcgaagaagtg5′-tccatatgtgagtgtggcagND+
BG9272815′-acgccaagctctaatacgac5′-ctcgggctatggtatatgacNDND
BI8254115′-accctggacaggtatggaat5′-accatgagcacagtgctcaaNDND
AA8884115′-ctcccattgcagttactatg5′-gtcgacatgttgttgaggtt++++
360H15AW5159665′-atgttcaccaggctggtctt5′-actaactctgtgggcttgcaNDND
BF8182195′-tctgcccactaacatctggt5′-cttgagtagctgagaccacaNDND
114C3No EST
432D3LOC144776D13S11905′-cacttccttgaaggggtttc5′-ctctgactcttgggcacaatND+++
AI1263135′-ttgagacacagtcctgctct5′-gcagggcacaatgttttcagNDND
319L6AV7310925′-attggtgaagccacctcaaa5′-caggctaacatggatctagtND+
BG9417145′-agtgcctacttcctaagacc5′-tcaggaatcagtgcccaaacND+
AI2629475′-ttgtgttcctggtccaccta5′-acattgggccggtcacttatND+
AI4931275′-tcaaatcaactgcacctcag5′-cagttcgagacttcttccatNDND
51B13BX0973355′-gaagtaggatggtgacacct5′-ctcagcaagtcatcctttgcNDND
430K10AL701000D13S8865′-tggcctggagtaattagctg5′-atttagggggtaggagagcaND+
LOC1608275′-ctgtgcactatcacttggga5′-taggctctaagccgttggttNDND
LOC1608265′-atggaatcaggttccctcca5′-ctgttcccttcatctgaatgNDND
BQ4773305′-caaagtgctgggattacagg5′-gccagctttgctgcacattaNDND
BQ4777415′-caacagaagatcggcccttt5′-actccctgaagcacagcattND+++
AV7318475′-caggcacttgcttaagggat5′-aactagcctgcttcagcttcNDND
BI4815225′-atgtgaagagaggtctcagc5′-agcaaaccacctagaggcttND+++
BU7292875′-cttagcctaatctccctaggcgga5′-tatcaggtaggtggtccagtctcaND++++++
BM7030785′-caggatcttgccctgttagt5′-tatcgggtggcgaacaagatND+++
AA7196725′-tgtccttaggtagacattgt5′-ttcaacctctgagaaacccaNDNDND
LOC1217345′-acttcactgtcaacagcgag5′-agagaccacatgcttgccatND++++++
BE4666875′-agttctggaggctaaaagtccagt5′-gccaaaatcacatggagagactacND+++
282D2BF3529935′-gagaacagtaatttctttcc5′-tgcaattattggggtaaagcNDNDND
BC0403205′-gtcatacacgtggacctaac5′-ctgaagtctcaagtgggcatND++++++
121J7AF3398285′-ctgacaagttctcagatcac5′-actctgcatgagcctagattND++++++
AA7019265′-agaccctgatggtctcttta5′-ggctcaatgttttcctacggND++++++
BF9080895′-tggaagaaaggacatgaggt5′-tctcatgaatccatgcccaaND+++
AW8684815′-aagtaaatgtgagaagtagc5′-tgctcatcctcattgtataND+
BX1073785′-taacctgagcagaatccagccttg5′-atggacccaaatgctgagaggaacND++++++
AA5990015′-aagagggacttgctgtgttg5′-tgcacagacggtacagaagtND+++++
GPC55′-cactggcgggtaaaggggac5′-agtattcagggaactgtcagtcacaccND+++
AL0436385′-ccagtctatcattgatggac5′-gaagtgcctctgtaattggaND++++++
AL7087345′-gtaatcccagcactttggga5′-tcttgttcttgtcccccagtND+++
487A2AF339802D13S14905′-tacctgggtaaccaagactc5′-ctctgttcactgcattgaagND++++++
H569195′-tgttgaccgactgagtgaac5′-ttatggtgaagtccttccccND++++
AA7054395′-cgtactctagagttaaccaa5′-atgattgtaagttccctgagND++++++
W868325′-atcctcatttctcaggggct5′-cctgtctgctctatgaagctND
N494425′-tggctgggcagaaatctgaa5′-tacaggtctgttcgccacatND++++++
N335965′-tccctagcaatgtgatgtac5′-ctaaggtattcctaggctcaND+
T849135′-gtagtaggtagaactgtcct5′-atctaccctcggcaattttcND++++++
BU6561345′-tgctagggctggagtacaat5′-cattttctcttggctcacccND++++++
93M14AW1054495′-ccagcaactgtaatacatgc5′-tcttcaaatccttgcctctgND+++
AV7546815′-acagccttctttggagagtg5′-tccaagggcacagtggaattND++++
  • a RT-PCR, reverse transcription-PCR; BAC, bacterial artificial chromosome; EST, expressed sequence tag; FB, fetal brain; ND, not done; STS, sequenced tagged site.

  • b Detection of a thin band from the result of electrophoresis.

  • c Detection of a thick band from the result of electrophoresis.