Table 1.

Inhibition of VEGF stimulation HMVEC migration

Peptides from K5IC50 values (nmol/L)
K5 (450-543)0.20 ± 0.01
Ac-VLLPDVETPSEED-NH2>1,000
Ac-MFGNGKGYRGKRATTVTGTP-NH2>1,000
Ac-QDWAAQEPHRHSIFTPETNPRAGLEKNY-NH2>1,000
Ac-RNPDGDVGGPW-NH20.50 ± 0.03
Ac-YTTNPRKLYDY-NH20.20 ± 0.02
Ac-DVPQ-NH2>1,000
Ac-RKLYDY-NH20.12 ± 0.02
Ac-KLYDY-NH20.10 ± 0.03
Ac-LYDY-NH2>1,000
Biotin-PRKLYDY0.23 ± 0.02
NH2-S-tag-K50.20 ± 0.05
125IK5 (dog)0.50 ± 0.08
  • NOTE: HMVECs were added to the bottom of a 48-well Boyden chamber and allowed to attach to the membrane for 2 hours ( 25). The chambers were inverted and medium containing 3 ng/mL VEGF and peptides was added to each well. The cells were incubated for 4 hours at 37°C in 5% CO2 to migrate. Finally, cells that did not migrate across the membranes were scraped off and the cells that migrated were stained and counted. IC50 values were determined by comparing migrated cells in nontreated wells with treated wells. Each IC50 value listed is an average of triplicates.