Table 1A.

Flow cytometric analysis of 7-AAD negatively gated, viable Colo−/Colo+ and CX−/CX+ carcinoma sublines

MarkerPlasma membrane–positive cells, % ± SE * (mean fluorescence intensity)
Colo−Colo+CX−CX+
Hsp7034 ± 5 (25 ± 5)73 ± 5 (29 ± 7)20 ± 6 (10 ± 4)90 ± 8 (18 ± 3)
Bag-429 ± 11 (24 ± 2)78 ± 1 (30 ± 5) 42 ± 10 (8 ± 1)78 ± 2 (17 ± 1)
MHC I99 ± 198 ± 298 ± 198 ± 2
  • * Data represent mean percentages of Hsp70 (cmHsp70.1), Bag 4 (IMG-152), and MHC I (W6/32) plasma membrane-positively stained cells of three independent experiments ± SE.

  • Significantly different from the corresponding negative cell type (P < 0.01).