Table 2A.

Specific lysis of Colo−/Colo+, CX−/CX+, and K562 target cells by differentially activated NK cells before and after blocking of the target cells with Hsp70 specific antibody cmHsp70.1

Target cellsMean values of specific lysis (% ± SE) *
ControlTKDCX− exosCX+ exosCX− lysCX+ lys
Colo−8 ± 319 ± 211 ± 618 ± 3ntnt
Colo+12 ± 428 ± 4 13 ± 530 ± 4 ntnt
CX−15 ± 543 ± 2.555 ± 450 ± 422 ± 620 ± 8
CX− plus cmHsp70.1nt43 ± 848 ± 941 ± 5ntnt
CX+19 ± 478 ± 5 62 ± 383 ± 3 23 ± 222 ± 8
CX+ plus cmHsp70.1nt20 ± 4 59 ± 443 ± 4 ntnt
K5625 ± 163 ± 8 20 ± 3.553 ± 7 32 ± 232 ± 2
K562 plus cmHsp70.1nt19 ± 8 25 ± 624 ± 8 ntnt
  • Abbreviations: lys, lysates; nt, not tested.

  • * Lysis of Colo−/Colo+, CX−/CX+, and K562 target cells at a distinct E/T ratio of 10:1, by CD94+ and CD3CD19 NK cells stimulated either with 100 IU/mL IL-2 only (control) or with a combination consisting of low-dose IL-2 (100 IU/mL) plus TKD (2 μg/mL; TKD), plus CX− exosomes/CX+ exosomes, or plus CX− lysates/CX+ lysates, at a concentration of 10 μg/mL each. Data represent mean values of at least three independent experiments ± SE.

  • Means significantly different from the relevant counterpart (P < 0.005).