Table 2C.

Cell surface density and fold increase in cell surface markers on CD56+ NK cells stimulated either with Hsp70 peptide TKD (2 μg/mL), CX−/CX+ exosomes (10 μg/mL), and lysates (10 μg/mL) derived thereof as compared to unstimulated NK cells

NK cell markerMean fluorescence intensity (fold increase)
TKDCX− exosCX+ exosCX− lysCX+ lys
CD94336 (2.0) *227 (1.3)315 (1.9) *241 (1.4)241 (1.4)
NKp30108 (2.6) *104 (2.4) *117 (2.7) *101 (2.4) *103 (2.4) *
NKp44266 (2.2) *293 (2.4) *313 (2.6) *256 (2.1)293 (2.4) *
NKp46342 (1.3)320 (1.2)354 (1.4)347 (1.3)333 (1.3)
NKG2D213 (1.1)276 (1.4)283 (1.4)273 (1.4)259 (1.3)
CD69185 (3.1) *139 (1.8)113 (2.4) *119 (1.8)118 (1.8)
CD158a524 (1.2)560 (1.2)505 (1.1)433 (1.0)442 (1.0)
  • NOTE: Data represent mean values of three independent experiments.

  • * Means significantly different from unstimulated controls. Similar results were obtained if CD3/CD19-depleted NK cells were used. The percentage of CD3/CD56+ NK cells in all experiments was always greater 85%.