Table 1.

ChoK efficiently potentiates RhoA-induced tumorigenicity

DNATumor incidence (%)Tumor volume (cm3)
PCDNAIIIb0/24 (0)0
RhoAQL8/24 (33)0.6 ± 0.4
ChoK8/30 (26)0.7 ± 0.4
RhoAQL/ChoK10/30 (33)2.7 ± 0.3
  • NOTE: Mice were injected s.c. with 1 × 106 of either Hek293T-pCDNAIIIb, Hek293T-RhoAQL, Hek293T-ChoKαa, or Hek293T-RhoAQL/ChoKαa transfected cells. Tumor growth was monitored weekly up to 50 days after injection. Whereas none of the control animals induce any detectable tumor, Hek293T-RhoAQL-, Hek293T-ChoKαa-, and Hek293T-RhoAQL/ChoKαa-transfected cells induced tumor growth in 26% to 33% of the cases. Both, tumor volume and growth rate of RhoAQL/ChoKαa cells were significantly higher than those of RhoAQL or ChoKαa cells. Differences were found significant between both RhoA and ChoKαa-transfected cells with respect to control cells (P ≤ 0.01) and between RhoA/ChoKαa expressing cells with respect to the expression of only RhoA or ChoKαa (P ≤ 0.001).