Table 2.

Real-time PCR verification for selected genes

GroupGAPDH ng total esophageal RNAData expressed as a ratio to ZS group after normalization to GAPDH
MT-1Adrenal secretory serine protease precursorEnolase 1 αRNLIPNTNFETUB
ZS1.9 ± 0.091.0 (reference)1.0 (reference)1.0 (reference)1.0 (reference)1.0 (reference)1.0 (reference)
ZD2.8 ± 1.037.0 ± 0.25.3 ± 0.042.7 ± 0.050.06 ± 0.020.23 ± 0.020.20 ± 0.01
ZR83.5 ± 0.7038 ± 0.33.9 ± 0.021.7 ± 0.010.05 ± 0.020.22 ± 0.020.22 ± 0.01
ZR122.8 ± 0.9718 ± 0.63.2 ± 0.021.4 ± 0.020.58 ± 0.020.31 ± 0.020.36 ± 0.01
ZR482.3 ± 0.502.8 ± 0.032.1 ± 0.020.8 ± 0.010.6 ± 0.020.37 ± 0.020.79 ± 0.01
  • NOTE: All results are presented as mean values from three to five esophagi.

    Abbreviations: ZD, zinc deficiency; ZR, zinc replenishment; ZS, zinc sufficiency.