Table 1.

Activin redistributes T47D cells into G0-G1 cell cycle arrest

T47DG0-G1SG2
Untreated70.2 ± 1.118.3 ± 0.611.5 ± 0.7
Activin A83.7 ± 1.67.8 ± 0.9 *8.5 ± 0.7 *
Follistatin60.6 ± 3.626.2 ± 2.1 *13.2 ± 1.5
Activin and follistatin58.2 ± 3.028.1 ± 1.9 *13.6 ± 1.2 *
  • NOTE: T47D cells were treated with activin A (100 ng/mL), follistatin (400 ng/mL), activin and follistatin combined, or TGF-β (10 ng/mL) for 48 hours in serum-free conditions and then stained with PI. DNA content was then analyzed by flow cytometry. Distribution of cells in the three phases of the cell cycle is represented by percentages. Representative means ± SD from three replicates of a representative experiment.

  • * Significant difference from untreated controls.