Table 1.

Fluorescence lifetimes of free (τ1) and protein-bound NADH (τ2) and the ratio of their relative contribution (a1:a2) at the three different cell confluences (25,000, 100,000, and 1,000,000) for n = 6 experimental control days with the averages and SD for any given imaging session (16 data points) and the combined average and SD for all imaging sessions (96 data points)

Confluence25,000100,0001,000,000
τ1 (ns) ± 0.05τ2 (ns) ± 0.1Ratio (a1:a2)τ1 (ns) ± 0.05τ2 (ns) ± 0.1Ratio (a1:a2)τ1 (ns) ± 0.05τ2 (ns) ± 0.1Ratio (a1:a2)
n = 10.4273.1371.2230.3662.5481.9170.3531.7252.750
n = 20.3882.9201.0150.3932.5881.7430.3551.7352.900
n = 30.4283.2090.9980.4202.6741.7970.3501.7532.929
n = 40.4553.1901.1670.4202.5862.0510.3461.7202.875
n = 50.4043.1141.3500.3982.5591.8440.3391.7082.300
n = 60.4333.0671.2980.3882.4341.9720.3581.7002.458
Average0.4233.1061.1750.3982.5651.8880.3501.7232.702
SD0.0230.1050.1450.0210.0780.1150.0070.0190.262