Table 2.

Fluorescence lifetimes of free (τ1) and protein-bound NADH (τ2) and the ratio of their relative contribution (a1:a2) at the three different cell confluences (25,000, 100,000, and 1,000,000) for 1, 3, 5, 7, and 10 minutes after addition of 1 mL of 4 mmol/L KCN with the averages and SD for all three imaging sessions combined (12 data points) and the combined average and SD for all time points and imaging sessions (60 data points)

Confluence25,000100,0001,000,000
Time lapsed (min)τ1(ns) ± 0.05τ2(ns) ± 0.1Ratio (a1:a2)τ1 (ns) ± 0.05τ2 (ns) ± 0.1Ratio (a1:a2)τ1 (ns) ± 0.05τ2 (ns) ± 0.1Ratio (a1:a2)
10.4232.052.10.3811.722.340.5032.053.41
30.3911.932.00.4011.912.410.4611.913.55
50.4112.152.20.3521.752.280.4221.823.72
70.3821.952.20.3611.822.330.4911.963.42
100.3852.072.00.3731.732.310.4731.883.82
Average0.3972.0242.1010.3721.7702.3340.4681.9223.574
SD0.0170.0990.0970.0190.0840.0980.0310.0870.174