Table 1.

Quantitation of testicular cells at different stages of development demonstrating apoptotic morphology following treatment of rats with doxorubicin or saline (control)

Age of donor animal at time of treatment (d)Stage of the seminiferous tubule segment examinedNumber of apoptotic cells (mm−1 tubule) *
24 h after treatment with48 h after treatment with
Saline (control)DoxorubicinSaline (control)Doxorubicin
6Total1 (0,4), n = 271 (1,5), n = 252 (0,5), n = 3119 (8,29), n = 31
    TUNEL1 (0,2)1 (0,6)1 (0,4)13 (8,23)
16Total7 (2,14), n = 508 (3,24), n = 508 (3,24), n = 367 (4,14), n = 37
    TUNEL9 (3,22)9 (5,29)7 (4,19)12 (15,21)
Stages II-VI3 (0,6), n = 132 (1,15), n = 122 (0,4), n = 62 (1,6), n = 15
Stages VII-VIII6 (2,10), n = 175 (3,13), n = 177 (2,21), n = 1110 (6,15), n = 10
Stages IX-XI13 (2,27), § n = 2517 (5,32), n = 2710 (3,26), n = 259 (4,16), n = 18
24Total8 (3,41), n = 559 (2,30), n = 4712 (2,28), n = 4021 (9,45), n = 39
    TUNEL12 (2,40)9 (2,23)11 (2,27)19 (9,33)
Stages II-VI3 (2,4), n = 123 (1,10), n = 128 (1,12), n = 164 (3,10), n = 7
Stages VII-VIII13 (6,46), n = 196 (3,29), n = 1015 (1,74), n = 2224 (15,44), n = 15
Stages IX-XI16 (7,45), n = 3011 (1,38), n = 3319 (7,36), n = 2023 (12,49), n = 19
  • NOTE: The median numbers of TUNEL-positive cells in the testicular samples are indicated.

  • * The values presented are median values of apoptotic cells (confidence limit: 25%,75%) of the numbers of samples examined (n).

  • P < 0.05, compared with the corresponding value for control testis from saline-treated rats at the same time point.

  • P < 0.05, compared with the corresponding value for stages II to VI in testis of 16-day-old doxorubicin-treated rats at the same time point.

  • § P < 0.05, compared with the corresponding value for stages II to VI in testis of 16-day-old control rats at the same time point.

  • P < 0.05, compared with the corresponding value for stages II to VI in testis of 24-day-old control rats at the same time point.