Table 1.

The replication capability and transgene expression of constructed armed oncolytic HSV-1 vectors in Vero cells (A) and mouse tumor cells (B)

(A)
VirusVirus yields (pfu)mIL-18 (pg/mL)B7-1-Ig expression
G47Δ2.0 × 107(−)
G47Δ-empty.17.5 × 106(−)
G47Δ-empty.26.1 × 106(−)
G47Δ-empty.36.3 × 106(−)
G47Δ-empty.47.2 × 106(−)
G47Δ-IL18/B7.17.6 × 1061,002(+)
G47Δ-IL18/B7.24.4 × 106818(+)
G47Δ-IL18/B7.38.3 × 106986(+)
G47Δ-IL18/B7.45.7 × 106338(+)
G47Δ-mIL-18.11.3 × 1071,418
G47Δ-mIL-18.23.2 × 106730
G47Δ-mIL-18.39.6 × 1061,710
G47Δ-mIL-18.47.6 × 106994
G47Δ-B7-1-Ig.11.6 × 107(+)
G47Δ-B7-1-Ig.23.9 × 106(+)
G47Δ-B7-1-Ig.31.2 × 107(+)
G47Δ-B7-1-Ig.45.8 × 107(+)
(B)
Virus and cellsmIL-18 (pg/mL)B7-1-Ig expression
G47Δ-IL18/B7 in Neuro2a538(+)
G47Δ-IL18/B7 in Pr14-2572(+)
G47Δ-IL18/B7 in TRAMP-C2806(+)
G47Δ-mIL-18 in Neuro2a483(−)
G47Δ-mIL-18 in Pr14-2658(−)
G47Δ-mIL-18 in TRAMP-C2673(−)