Table 1.

Quantitative assessment of CaSR immunoreactivity in normal colonic crypt epithelium and colon carcinoma.

Histological features% Positive cellsStaining intensity
Normal colonic crypt epithelium (n = 9)
Basal zone6 ± 11.1 ± 0.1
Mid-crypt90 ± 5 *2.3 ± 1.5
Surface96 ± 2 *2.9 ± 1.0 *
Well- to moderately differentiated tumor (n = 7)63 ± 131.7 ± 0.7
Poorly to undifferentiated tumor (n = 4)3 ± 1 1.1 ± 0.1
Cells at invasive front (n = 2)5 ± 1 1.4 ± 0.1
  • NOTE: Normal colon. Basal zone: base of the crypt containing small, densely packed cells without evidence of goblet cell structure. Mid-crypt: area from the edge of the basal zone to an area 1/3 of the way from the top of the crypt. Surface zone: upper 1/3 of the crypt and the cells at the apex of the crypt. Values are means and standard errors based on four separate areas in each specimen.

    Colon carcinoma. Well- to moderately differentiated tumor: evidence of glandular structure. Poorly to undifferentiated tumor: areas in which only cell sheets were detected (i.e., no glandular structure). Invasive front: small clusters of tumor cells (1-3 cells) imbedded in the stroma. Values are means and standard errors based on four separate areas in each specimen.

  • * P < 0.01 statistically significant difference from basal zone.

  • P < 0.05, statistically significant difference from basal zone.

  • P < 0.01, statistically significant difference from well- to moderately differentiated tumor.