Table 2.

Inhibition of tumor formation by down-regulation of SP1 expression in patient-derived fibrosarcoma cell line SHAC

SHACRelative Sp1 level *Frequency (tumors/sites)Latency (wk)
Parental1.06/69
V10.83/46-8
V21.03/49-11
SpR30.65/1211-17
SpR10.43/1018-30
SpR20.41/1217
  • NOTE: Cells (106) were injected s.c. into the right and left rear flank regions of athymic mice (two sites per mouse). Mice were examined for tumors weekly. When tumors reached ∼1 cm in diameter, they were removed. The data in parentheses are from a second experiment with the same cell lines. V, vector control; SpR, transfectants expressing Sp1 U1snRNA/Ribozyme.

  • * Relative Sp1 level determined by Western blotting.

  • Time required for tumors to reach ∼1 cm.