OTX2 expression in medulloblastomas
| Sample ID | Diagnosis * | Fold of OTX2 expression by Q-PCR † | OTX2 SAGE tag numbers per 200,000 tags ‡ |
|---|---|---|---|
| Normal cerebellum | 1.00 | NA | |
| Medulloblastoma cell lines | |||
| D487 | NA | 128.00 | NA |
| D721 | NA | 90.62 | NA |
| D425 | NA | 72.17 | NA |
| D283 | NA | 56.76 | 54 |
| D384 | Anaplastic, large cell | 42.83 | NA |
| D556 | Anaplastic, large cell | 35.59 | NA |
| D458 | NA | 35.03 | NA |
| D341 | NA | 13.10 | 72 |
| D581 | NA | 0.91 | NA |
| D324 | NA | 0.10 | NA |
| MHH1 | NA | 0.01 | 0 |
| MCD-1 | NA | 0.01 | NA |
| UW228 | NA | 0.00 | NA |
| Medulloblastoma primary tumors | |||
| TB1244 | Anaplastic, large cell | NA | 77 |
| TB285 | Anaplastic | NA | 45 |
| TB1273 | Anaplastic | NA | 31 |
| TB476 | Anaplastic, large cell | 328.43 | NA |
| TB2235 | Classic, with focal anaplasia | 124.68 | NA |
| TB2227 | Anaplastic | 100.50 | NA |
| TB2223 | Desmoplastic, nodular | 85.36 | NA |
| TB2226 | Anaplastic | 57.57 | NA |
| TB1377 | Anaplastic, large cell | 54.05 | NA |
| TB1961 | Anaplastic, large cell | 53.68 | NA |
| TB830 | Classic | 44.09 | NA |
| TB54 | Classic, with focal anaplasia | 37.93 | 22 |
| TB1339 | Anaplastic, large cell | 36.49 | 9 |
| TB2178 | Desmoplastic, with focal anaplasia | 18.10 | NA |
| TB771 | Anaplastic, large cell | 3.19 | 0 |
| TB2224 | Desmoplastic, nodular | 1.52 | NA |
| TB2222 | Desmoplastic, nodular | 0.91 | NA |
| TB1341 | Desmoplastic, nodular | 0.31 | NA |
| TB2025 | Desmoplastic | 0.18 | NA |
| TB876 | Desmoplastic, nodular | 0.17 | 0 |
| TB100 | Classic | 0.05 | 0 |
| TB2228 | Classic | 0.02 | NA |
| TB1423 | Desmoplastic | 0.00 | NA |
Abbreviation: NA, data not available.
↵* Histopathologic categories were defined by two board-certified neuropathologist (REM, TJP) at Duke as follows. Desmoplasia: connective tissue and/or pericellular reticulin fibers distributed throughout the tumor. Large cell: cells with large open nuclei with or without enlarged nucleoli. Anaplasia: high mitotic activity, nuclear crowding, extensive apoptosis and necrosis, or extensive large cell change. Classic pattern: low to moderate mitotic activity, apoptotic activity, and necrotic foci.
↵† Fold of OTX2 expression was defined as the ratio of OTX2 expression in tumor relative to normal.
↵‡ The SAGE data were obtained from the National Center for Biotechnology Information Cancer Genome Anatomy Project repository and the OTX2 SAGE tag, and its presence in the tumors was identified by using the SAGE Anatomic Viewer.