Table 1.

Comparison of the timing of PPARγ inhibitor effects on adhesion and on measurements of apoptosis and cell death

Time (h)Morphology% DNA in subdiploid peak% Cells early apoptosis% Cells Annexin V+% Cells dead
17
    ControlMixed rounded and flattened3.3 ± 0.66.9 ± 0.318 ± 212 ± 6
    Poly-HEMANot adherent13 ± 515 ± 238 ± 310 ± 4
    T0070907Rounded3.9 ± 0.48.5 ± 0.320 ± 110 ± 5
    GW9662Mixed rounded and flattened3.5 ± 0.57.9 ± 0.921 ± 213 ± 5
40
    ControlFlattened2.4 ± 0.410 ± 326 ± 720 ± 10
    Poly-HEMANot adherent9 ± 312 ± 328 ± 657 ± 9
    T0070907Not adherent12 ± 111 ± 224 ± 622 ± 4
    GW9662Rounded3 ± 118 ± 234 ± 319 ± 6
67
    ControlFlattened3 ± 36.7 ± 0.510 ± 14 ± 1
    Poly-HEMANot adherent7 ± 49 ± 320 ±576 ± 6
    T0070907Not adherent9.7 ± 0.124 ± 228 ± 227 ± 4
    T0070907 +ZNot adherent1.3 ± 0.45.1 ± 0.57 ± 16 ± 3
    GW9662Not adherent12 ± 427 ± 333 ± 24 ± 1
88
    ControlFlattened5 ± 2
    T0070907Not adherent24 ± 4
    GW9662Not adherent24 ± 2
>120
    Control flattened7 ± 9
    T0070907Not adherent94 ± 7
    GW9662Not adherent89 ± 16
  • NOTE: Data corresponds to images shown in Fig. 2, with the percentage of cells in early apoptosis (Annexin V+ PI) and in both early and late apoptosis (total Annexin V+ = Annexin V+ PI and Annexin V+ PI+) determined using the quadrants as shown. Poly-HEMA denotes control cells placed on plates coated with polyhydroxyethylmethacrylate, which prevents all cell adhesion to the plate. Cells pretreated with the pan-caspase inhibitor z-VAD-FMK for 2 hours before addition of T0070907 (Z). Cell death was assessed using trypan blue exclusion. Replicate standard deviations (n = 3). Representative of at least three independent experiments.