Table 1.

Cellularity and cellular composition of various draining lymph nodes during tumor progression

WT (×106)Transgenic (×106)
Lymph nodes *Submandibular7.3 ± 3.58.7 ± 3.6
Inguinal5.4 ± 2.310.0 ± 2.3
Mesenteric8.4 ± 4.713.7 ± 4.4
Lumbar1.8 ± 0.810.5 ± 4.0
Lumbar lymph nodes11-12 wk1.9 ± 0.43.2 ± 0.6
16-17 wk1.7 ± 0.612.8 ± 1.7
20-25 wk1.8 ± 0.810.5 ± 4.0
Lumbar lymph nodes (20-25 wk)CD11c z0.0234 ± 0.00670.234 ± 0.085
CD40.926 ± 0.3684.56 ± 0.71
CD80.825 ± 0.1702.61 ± 0.26
  • * Cell number is per lymph node isolated from mice ≥20 weeks of age.

  • Statistically different (P ≤ 0.01) versus WT as determined using the Student's t test.

  • Absolute numbers of cells expressing CD11c, CD4, and CD8 in each lumbar lymph node were calculated by multiplying total lumbar lymph node cellularity with the percentage of viable nucleated cells expressing the indicated markers as determined by flow cytometry. Each value represents the mean ± SD (n ≥ 3 individual experiments).