Table 1.

Effect of D6.1A-expressing tumor cells on surrounding cells and tissue

MarkerControl % stained (intensity)AS, i.p.AS-D6.1A, i.p.
% Stained (intensity)P value control, % (intensity) *% Stained (intensity)P value control, % (intensity)P value AS, % (intensity)
Peritoneal exudate macrophages
    Integrins
        αM97 (243)97 (1,254)— (<0.0001)98 (1,841)— (0.0002)— (0.0100)
        α312 (30)25 (138)0.0334 (0.0011)26 (177)0.0360 (0.0003)
        α492 (90)97 (598)— (<0.0001)97 (580)— (<0.0001)
        α580 (122)81 (582)— (0.0019)82 (633)— (0.0019)
        α624 (25)33 (35)36 (25)
        β272 (347)99 (1,155)— (0.0024)99 (1,864)— (0.0002)— (0.0122)
        β338 (17)55 (48)0.0276 (0.0047)73 (72)0.0026 (0.0005)0.0407 (0.0213)
    Tetraspanins
        CD987 (69)98 (635)— (<0.0001)97 (658)— (<0.0001)
        CD8161 (134)91 (696)0.0312 (<0.0001)89 (816)0.0354 (<0.0001)
        CD15136 (18)54 (92)0.0313 (0.0012)56 (98)0.0344 (0.0020)
        D6.1ANot detectable16 (503)0.0080 (<0.0001)32 (563)0.0010 (<0.0001)0.0238 (—)
    Angiogenic factors
        VEGF12 (14)27 (31)0.0154 (0.0384)41 (30)0.0034 (0.0422)0.0479 (—)
        bFGF13 (15)46 (69)0.0045 (0.0063)53 (63)0.0024 (0.0052)
        TNFα37 (96)59 (118)0.0288 (—)72 (132)0.0077 (—)
    Matrix-degrading enzymes and inhibitors
        MMP-228 (19)38 (52)— (0.0101)52 (48)0.0213 (0.0126)
        MMP-914 (13)47 (81)0.0011 (0.0212)47 (71)0.0019 (0.0256)
        MMP-1322 (15)31 (43)— (0.0103)38 (46)0.0425 (0.0090)
        TIMP-16 (10)18 (23)0.0315 (0.0445)17 (26)0.0409 (0.0389)
        TIMP-24 (11)12 (15)0.0363 (—)19 (15)0.0085 (—)
        uPA2 (11)8 (15)15 (40)0.0070 (0.0090)— (0.0084)
        uPAR9 (13)17 (25)— (0.0424)30 (56)0.0066 (0.0046)0.0334 (0.0161)
Mesenteric cells
    Integrins
        αM9 (129)76 (601)0.0003 (0.0002)68 (789)0.0004 (0.0004)— (0.0436)
        α14 (37)44 (201)0.0027 (0.0009)30 (183)0.0062 (0.0013)
        α222 (20)36 (79)0.0361 (0.0013)37 (92)0.0292 (0.0012)
        α350 (31)87 (182)0.0060 (0.0035)78 (281)0.0068 (0.0005)— (0.0184)
        α436 (147)39 (127)64 (122)0.0123 (—)0.0206 (—)
        α574 (91)70 (193)— (0.0080)74 (217)— (0.0038)
        α659 (114)68 (128)66 (134)
        β145 (23)72 (55)0.0154 (0.0105)74 (147)0.0151 (0.0022)— (0.0073)
        β286 (135)42 (258)0.0025 (0.0275)55 (230)0.0138 (0.0406)
        β335 (90)30 (74)33 (104)
    Tetraspanins
        CD989 (312)87 (198)— (0.0276)86 (186)— (0.0271)
        CD8195 (412)77 (556)— (0.0241)75 (760)— (0.0042)— (0.0265)
        CD15194 (115)89 (272)— (0.0132)78 (165)
        D6.1A21 (67)33 (73)41 (172)— (0.0345)— (0.0284)
    Angiogenic factors
        VEGF37 (35)42 (61)— (0.0388)70 (217)0.0008 (0.0016)0.0009 (0.0029)
        bFGF46 (81)58 (134)— (0.0225)76 (322)— (0.0015)0.0128 (0.0050)
        TNFα65 (105)69 (121)65 (112)
    Antiangiogenic factors
        IFNγ39 (50)8 (37)0.0055 (—)11 (31)0.0092 (—)
        IL447 (81)29 (35)0.0277 (0.0098)25 (31)0.0309 (0.0073)
        TIMP-159 (50)27 (49)0.0168 (—)32 (39)0.0222 (—)
        TIMP-249 (64)42 (46)46 (52)
    Matrix-degrading enzymes
        MMP-215 (31)27 (67)0.0472 (0.0116)48 (212)0.0090 (0.0006)0.0021 (0.0014)
        MMP-913 (30)27 (40)0.0235 (—)47 (140)0.0198 (0.0043)0.0032 (0.0067)
        MMP-1313 (52)17 (134)— (0.0032)29 (178)0.0500 (0.0023)0.0113 (—)
        uPA15 (68)20 (57)39 (294)0.0267 (0.0001)0.0281 (0.0001)
        uPAR39 (90)32 (93)38 (205)— (0.0101)— (0.0094)
    Endothelial cell markers
        CD3143 (137)48 (113)65 (298)0.0211 (0.0066)0.0234 (0.0053)
        VEGFR123 (44)34 (47)54 (703)0.0213 (0.0003)0.0311 (0.0004)
        VEGFR231 (53)45 (47)58 (102)0.0381 (0.0192)0.0431 (0.0109)
  • * Nonsignificant differences (P > 0.05) are indicated by a dash.