Table 1.

Target sequences of PBK/TOPK inserted into siRNA expression vector

psiU6BX3.0-si-#1
    5′-GGCAGACATATTTGCCTTT-3′
psiU6BX3.0-si-#2
    5′-CTGGATGAATCATACCAGA-3′
psiU6BX3.0-si-#3
    5′-GTGTGGCTTGCGTAAATAA-3′
psiU6BX3.0-mis-#1 *
    5′-cTGTGGCTTcCGTAAATAt-3′
psiU6BX3.0-mis-#2 *
    5′-cTGTGGCTTaCGTAAATAt-3′
psiU6BX3.0-mis-#3 *
    5′-cTGTGGCTTcCGaAAATAt-3′
  • NOTE: Underlined bases indicate siRNA-targeting sequences designed from PBK/TOPK mRNA (Genbank accession no. NM018492).

  • * Mismatched oligonucleotides were designed from si-3 by substitution of some internal bases (indicated by small letters).