Table 1.

Arachidonic acid significantly induces production of PGE2 as early as 5 minutes

PGE2 concentration (pg/mL)
Control13.1 ± 6.4
AA38.9 ± 8.1 *
AA + F12.9 ± 2.6
AA + Indo13.5 ± 8.0
  • NOTE: Arachidonic acid (5 μg/mL) was added to down-regulated PC-3 cells. Cells (1 × 106) were seeded in each well. Three hours before addition of arachidonic acid, 25 μmol/L of flurbiprofen or 25 μmol/L of indomethacin were added to the cells. At 5 minutes after addition of arachidonic acid, medium was collected and PGE2 enzyme immunoassay was done (n = 3).

    Abbreviations: AA, arachidonic acid; F, flurbiprofen; Indo, indomethacin.

  • * P < 0.05 against control samples.

  • P < 0.01 against arachidonic acid samples with two-tailed Student's t test.

  • P < 0.05 against arachidonic acid samples with two-tailed Student's t test.