Table 2.

Expression of insulin-related proteins and BrdUrd index in preneoplasias and hepatocellular tumors

CCFMCFBCFcHCAbHCAcHCCbHCC
Insulin receptor
    Membranous↓(=)= (↓)=====
    Intracytoplasmic↑ (=)= (↓)↑↑= (↓)↑↑= (↓)
IRS-1↑↑↑ (=)= (↓)↑↑= (↓)↑↑= (↓)
Raf-1↑↑↑ (=)= (↓)↑↑= (↓)↑↑= (↓)
Mek-1↑↑↑ (=)= (↓)↑↑= (↓)↑↑= (↓)
IGF-I↑↑==↓ (=)
IGF-I receptor===↑ (=)↑ (=)↑ (=)↑ (=)
IGF-II/M6P receptor↑↑↑↑↑↑
TGF-α=↑↑↑↑↑↑
GST-P=↑ (=)↑ (=)↑↑↑↑
Glucose-6-phosphatase↓↓N.D.N.D.N.D.N.D.N.D.
Fatty acid synthase *↑↑N.D.N.D.N.D.N.D.N.D.N.D.
Increase of BrdUrd labeling index 39 ± 415 ± 625 ± 336 ± 468 ± 565 ± 480 ± 6
  • NOTE: The intensity of the immunohistochemical variables in focal lesions was estimated semiquantitatively compared with unaltered liver tissue of the same sections using the following grades: ↓↓, strong decrease; ↓, decrease; = equal; ↑, increase; ↑↑, strong increase. If staining was not uniform in one lesion, the secondary pattern is given in parentheses.

    Abbreviations: BCF, basophilic cell focus; cHCA/cHCC, clear cell HCA/clear cell HCC; bHCA/bHCC, basophilic HCA/basophilic HCC; GST-P, glutathione S-transferase (placental form); N.D., not determined.

  • * Data obtained from a previous study (see ref. 23).

  • X-fold increase when compared with the adjacent unaltered liver tissue.