Table 2.

Soft agar colony formation assay for HBEC3 cells treated with gefitinib or erlotinib

HBEC3
Vectorp53RNAiK-RASV12p53RNAi and K-RASV12
EGF(+)
    DMSO1.3 ± 0.4711 ± 1.65.7 ± 1.223 ± 2.1
    Gefitinib (1 μmol/L)0000
    Erlotinib (1 μmol/L)0000
EGF(−)
    DMSO0001.3 ± 0.94
    Gefitinib (1 μmol/L)0000
    Erlotinib (1 μmol/L)0000
  • NOTE: Number of colonies after 14 days are shown as average ± SD. HBEC3 cells (1,000) were plated in agar and treated with 1 μmol/L gefinitib or erlotinib in triplicate 12-well plates for up to 2 weeks in the presence or the absence of EGF and colonies (50-100 cells) were counted.