Table 1.

Immunogenetic features in breast cancer cell lines at whole population and single-cell level

Whole population
IgMIgGIgA
VHJH%HVHJH%HVHJH%H
MDA-MB-231V3-744b95.3NDND
SKBR3NDV4-30.16c96.7V3-736b89.7
T47DNDV3-414b *ND
ZR75-1V5-516c100V3-235b91.9ND
BT474NDNDND
MCF-7NDNDND
Single-cell level
VH%HSingle cells
No. cells positive for VH/totalNo. cells positive for AID/total
MDA-MB-231V3-7495.37/2411/24
T47DV3-41 8/2410/24
ZR75-1V5-511003/246/24
V3-2391.95/248/24
  • NOTE: Clones (24-28) for each V(D)J rearrangement were sequenced to examine intraclonal variation. Rearranged V(D)J sequences have been deposited in Genbank (accession no. DQ379471-6).

    Abbreviations: %H, homology to the germ-line gene at the nucleotide level; ND, not detected.

  • * V3-41 is a pseudogene with two stop codons in the germ-line gene.

  • All VH-positive cells were confirmed as expressing AID transcripts.