Table 3.

Stimulation of human PBMCs by hLAG-3Ig induces the release of an activating pattern of proinflammatory cytokines and chemokines

Cytokine/chemokine release (pg/mL)
IFN-γTNF-αIL-1βIL-4IL-6RANTES (CCL5)MIG (CXCL9)IP-10 (CXCL10)
/2755118821,254798>10,000>10,000
+hLAG-3Ig465,6242,871654>10,0003,9631,0512,506
  • NOTE: Results of Th1/Th2, inflammation, and chemokine CBA assays of supernatants from antigen-stimulated healthy donors' PBMCs cultured for 48 hours either alone or in the presence of 16 μg/mL hLAG-3Ig. Data reported are representative of experiments done on three healthy donors and indicate the quantitative measurement of cytokines or chemokines expressed as pg/mL. Assay sensitivity = 10 pg/mL. SDs calculated on three replicates were <10% of the indicated value.