Table 1.

Fraction of cloned PCR products with indicated mutations

CellsNo. of clones sequenced (%)
Wild-type *15-bp deletion *15-bp del+T790M *T790M
PC-96/10 (60%)4/10 (40%)0/10 (0%)
RPC-99/29 (31%)12/29 (41%)8/29 (27%)
RPC-9-36d0/20 (0%)
RPC-9-44d0/20 (0%)
RPC-9-55d1/40 (2.5%)
  • * PCR was carried out from cDNA, and products were subcloned and sequenced.

  • EGFR exon 20 was amplified from genomic DNA, and products were subcloned and sequenced.