Table 1.

Kinetic analysis of UGT1A4-, UGT2B10-, and HLM-induced glucuronidation of nicotine and cotinine

NicotineCotinine
KM (mmol/L)Vmax (pmol·min−1·mg protein−1)KM (mmol/L)Vmax (pmol·min−1·mg protein−1)
UGT1A417.5 ± 2.6133 ± 710.3 ± 1.371.8 ± 2.5
UGT2B100.47 ± 0.2611.5 ± 2.23.5 ± 0.1925 ± 1.1
HLM0.93 ± 0.2643 ± 315.5 ± 1.11,144 ± 85
  • NOTE: Data are expressed as mean ± SD for three independent experiments.