Table 1.

Drug accumulation levels in the βIII-tubulin siRNA-treated Calu-6 and H460 NSCLC cells

TransfectionMean [3H]paclitaxel uptake (pmol/mg of protein)Mean [3H]vincristine uptake (pmol/mg of protein)
0 hP *2 hP *0 hP *2 hP *
Drug uptake by siRNA-transfected NSCLC Calu-6 cells
    Mock1.84 ± 0.25(Referent)39.3 ± 2.43(Referent)0.56 ± 0.11(Referent)16.1 ± 1.21(Referent)
    Control siRNA1.87 ± 0.030.9144.9 ± 2.040.150.39 ± 0.080.2515.4 ± 0.980.66
    βIII-Tubulin siRNA2.05 ± 0.280.5939.6 ± 2.80.940.31 ± 0.070.1014.8 ± 0.880.43
Drug uptake by siRNA-transfected NSCLC H460 cells
    Mock2.609 ± 0.299(Referent)52.898 ± 1.722(Referent)0.267 ± 0.075(Referent)8.502 ± 0.336(Referent)
    Control siRNA3.576 ± 0.7760.3153.868 ± 2.8410.780.312 ± 0.0660.678.631 ± 0.3970.82
    βIII-Tubulin siRNA2.781 ± 0.8260.8553.898 ± 1.5790.690.310 ± 0.0110.598.683 ± 0.8590.85
  • NOTE: Data are from at least three independent experiments.

  • * Student's t test (two-sided).