Table 3.

Gene induction in various tissues of mice (p53+/+) after gamma-irradiation

(A)
FunctionTissueTSC2PTENIGF-BP3p21
8 h24 h8 h24 h8 h24 h8 h
Energy metabolismSkeletal muscle2.6 ± 0.62.0 ± 0.62.6 ± 0.72. 3 ± 0.82.5 ± 0.62.1 ± 0.43.6 ± 0.3
Heart1.8 ± 0.42.1 ± 0.62.1 ± 0.51.9 ± 0.61.8 ± 0.31.6 ± 0.42.8 ± 0.3
White fat1.4 ± 0.42.8 ± 0.71.4 ± 0.62.7 ± 0.80.8 ± 0.31.2 ± 0.34.6 ± 0.5
Liver1.6 ± 0.21.8 ± 0.22.1 ± 0.62.0 ± 0.31.2 ± 0.30.9 ± 0.28.6 ± 1.3
Kidney1.9 ± 0.41.5 ± 0.52.0 ± 0.51.6 ± 0.50.9 ± 0.20.7 ± 0.312.0 ± 0.9
OthersSpleen1.2 ± 0.31.3 ± 0.41.2 ± 0.21.3 ± 0.32.4 ± 0.34.1 ± 0.822.7 ± 1.5
Thymus0.7 ± 0.21.1 ± 0.40.7 ± 0.21.1 ± 0.24.2 ± 0.86.6 ± 0.715.8 ± 1.6
Small intestine0.8 ± 0.21.1 ± 0.30.9 ± 0.21.4 ± 0.42.8 ± 0.63.7 ± 0.54.2 ± 0.6
Colon1.3 ± 0.21.6 ± 0.41.4 ± 0.31.5 ± 0.30.9 ± 0.31.6 ± 0.32.8 ± 0.3
Lung1.7 ± 0.51.4 ± 0.22.1 ± 0.61.5 ± 0.31.4 ± 0.32.0 ± 0.42.9 ± 0.4
Skin0.8 ± 0.20.9 ± 0.151.3 ± 0.21.2 ± 0.150.8 ± 0.30.9 ± 0.22.5 ± 0.3
Brain cortex1.3 ± 0.31.1 ± 0.21.3 ± 0.21.1 ± 0.21.8 ± 0.41.2 ± 0.33.5 ± 0.5
(B)
FunctionTissueMDM2Pirh2Cop1Cyclin G1Wip1
8 h24 h8 h24 h8 h24 h8 h24 h8 h24 h
Energy metabolismSkeletal muscle3.0 ± 0.72.5 ± 1.02.4 ± 0.63.0 ± 0.71.2 ± 0.31.1 ± 0.24.8 ± 1.33.6 ± 0.72.7 ± 0.82.1 ± 0.4
Heart3.2 ± 0.42.6 ± 0.42.0 ± 0.42.2 ± 0.51.3 ± 0.31.4 ± 0.21.8 ± 0.52.1 ± 0.41.3 ± 0.21.3 ± 0.4
White fat3.1 ± 0.72.6 ± 0.82.2 ± 0.62.6 ± 0.81.2 ± 0.31.3 ± 0.54.6 ± 0.84.2 ± 1.21.5 ± 0.41.2 ± 0.3
Liver3.6 ± 0.52.8 ± 0.42.6 ± 0.32.7 ± 0.41.4 ± 0.31.6 ± 0.45.3 ± 1.22.3 ± 0.72.8 ± 0.91.4 ± 0.5
Kidney2.8 ± 0.42.6 ± 0.62.6 ± 0.52.1 ± 0.51.3 ± 0.21.5 ± 0.64.5 ± 0.92.4 ± 0.42.2 ± 0.61.2 ± 0.3
OthersSpleen6.8 ± 1.14.8 ± 0.80.8 ± 0.30.8 ± 0.20.6 ± 0.30.7 ± 0.46.5 ± 1.85.7 ± 1.42.3 ± 0.41.8 ± 0.3
Thymus4.5 ± 0.93.6 ± 0.70.9 ± 0.21.2 ± 0.30.8 ± 0.20.6 ± 0.24.2 ± 0.83.6 ± 0.71.3 ± 0.40.8 ± 0.3
Small intestine2.1 ± 0.32.2 ± 0.50.8 ± 0.21.3 ± 0.31.4 ± 0.51.1 ± 0.32.3 ± 0.41.8 ± 0.41.2 ± 0.31.6 ± 0.5
Colon2.8 ± 0.82.3 ± 0.81.3 ± 0.31.5 ± 0.41.3 ± 0.40.9 ± 0.35.2 ± 0.94.7 ± 1.12.5 ± 0.72.8 ± 0.8
Lung3.2 ± 12.4 ± 0.42.0 ± 0.71.2 ± 0.21.3 ± 0.51.2 ± 0.25.6 ± 0.83.3 ± 0.62.5 ± 0.61.4 ± 0.3
Skin2.1 ± 0.42.3 ± 0.51.5 ± 0.31.7 ± 0.30.8 ± 0.31.3 ± 0.21.8 ± 0.42.0 ± 0.52.0 ± 0.41.2 ± 0.4
Brain cortex2.0 ± 0.31.8 ± 0.21.3 ± 0.21.4 ± 0.20.8 ± 0.21.1 ± 0.22.5 ± 0.61.7 ± 0.41.3 ± 0.30.9 ± 0.3
  • NOTE: C57BL/6 p53+/+ mice were treated with 5 Gy gamma-irradiation and sacrificed at 8 and 24 h postirradiation. The RNA levels of different genes were determined by TaqMan quantitative real-time PCR. All expression levels were normalized to actin. Gene induction was calculated as gene expression levels in irradiated mice compared with nonirradiated mice. No clear induction was observed for AMPK β1 in any tissues.