Table 1.

Sequence of mutagenesis primers

Mutation siteSequence of primer
K69Q5-GAA AGC TTG ACA GAT CCC AGT CAA TTA GAC TCT GGG A
K69R5-GAA AGC TTG ACA GAT CCC AGT AGA TTA GAC TCT GGG A
K100Q5-GAT ACT GGA ATT GGA ATG ACC CAG GCT GAC TTG ATC
K100R5-GAT ACT GGA ATT GGA ATG ACC AGG GCT GAC TTG ATC
K292Q5-TCG ATC AAG AAG AGC TCA ACC AAA CAA AGC CCA TCT G
K292R5-TCG ATC AAG AAG AGC TCA ACA GAA CAA AGC CCA TCT G
K327Q5-TGG GAA GAT CAC TTG GCA GTG CAG CAT TTT TCA GTT G
K327R5-TGG GAA GAT CAC TTG GCA GTG AGG CAT TTT TCA GTT G
K478Q5-GTG ATG AGA TGG TTT CTC TCC AGG ACT ACT GCA CCA G
K478R5-GTG ATG AGA TGG TTT CTC TCA GGG ACT ACT GCA CCA G
K546Q5-GAA GAC TTT AGT GTC AGT CAC CCA AGA AGG CCT GGA ACT
K546R5-GAA GAC TTT AGT GTC AGT CAC CAG AGA AGG CCT GGA ACT
K5585-TCC AGA GGA TGA AGA AGA GCA AAA GAA GCA GGA AG
K558R5-TCC AGA GGA TGA AGA AGA GAG AAA GAA GCA GGA AG