Table 2.

Selected gene expression changes in HL-60 cells treated with 6 μmol/L CHR-2797 or deprived of extracellular amino acids

Gene symbolDescriptionCHR-2797 6 hCHR-2797 24 hw/o AAs 6 h
CLEC7AC-type lectin domain family 7, member A6.729.56.3
STC2Stanniocalcin 213.315.33.7
CCNG2Cyclin G25.113.94.5
DDIT4DNA-damage-inducible transcript 4 (REDD1)18.713.84.0
CDKN1ACyclin-dependent kinase inhibitor 1A (p21, Cip1)1.711.73.0
CTHCystathionine γ-lyase17.911.31.7
PPP1R15AProtein phosphatase 1, regulatory (inhibitor) subunit 15A (GADD34)
ATF5Activating transcription factor
TRIB3Tribbles homologue
MGC4504Hypothetical protein11.96.05.1
ATF3Activating transcription factor
VEGFVascular endothelial growth factor5.85.42.3
FYNProtooncogene tyrosine protein kinase Fyn4.65.12.4
ASNSAsparagine synthetase4.45.11.8
DDIT3DNA-damage-inducible transcript 3 (CHOP, GADD153)
WARSTryptophanyl-tRNA synthetase1.94.21.2
SLC7A11Solute carrier family 7, (cationic amino acid transporter, y+ system) member
ASSArgininosuccinate synthetase1.63.70.7
CBSCystathionine β-synthase3.83.41.6
SLC38A2Solute carrier family 38, member 2 (SNAT2)
GADD45AGrowth arrest and DNA-damage-inducible, α (DDIT1)
PSAT1Phosphoserine aminotransferase
CARSCysteinyl-tRNA synthetase2.22.02.8
CEBPBCCAAT/enhancer binding protein (C/EBP), β1.21.92.9
SARSSeryl-tRNA synthetase1.61.91.1
MARSMethionine-tRNA synthetase2.11.41.4
  • NOTE: HL-60 cells were treated with 6 μmol/L CHR-2797 for 6 and 24 h or deprived of extracellular amino acids for 6 h. Gene expression was measured by real-time quantitative PCR and compared with cells treated with 0.06% (v/v) DMSO (for CHR-2797 treatment) or cells grown in normal growth medium supplemented with dialyzed FBS (for amino acid deprivation). Results for CHR-2797 were generated from the same pooled triplicate samples used for the gene expression microarray experiments, and those for amino acid deprivation are representative of at least three independent experiments.

    Abbreviation: w/o AAs, deprived of extracellular amino acids.