Table 1.

CCR6 distribution among TIL- and PBL-derived T-cell clones and uncultured TIL and peripheral blood mononuclear cells

CCR6 expression, % (mean fluorescence intensity)
TIL clones (CD8+)
    Heu171 (αEβ7+)90 (42)
    Heu127 (αEβ7+)80 (26)
    Heu161 (αEβ7+)64 (23)
PBL clones (CD8+)
    H32-22 (αEβ7)4
    H32-8 (αEβ7+)10 (50)
    H32-25 (αEβ7+)7 (22)
CCR6 expression on CD8+ fraction, % (mean fluorescence intensity)
Uncultured TIL
    Patient 228 (274)
    Patient 439 (96)
    Patient 534 (135)
    Patient 649 (299)
    Patient 747 (300)
Mean, 39 ± 9
Uncultured peripheral blood mononuclear cells
    Patient 1 (Heu)1
    Patient 515 (129)
    Patient 61
    Patient 710 (141)
    Patient 820 (55)
    Patient 93
Mean, 8 ± 8
Uncultured peripheral blood mononuclear cells
    Donor 118 (113)
    Donor 212 (87)
    Donor 314 (51)
    Donor 48 (57)
    Donor 514 (104)
    Donor 614 (85)
    Donor 72
Mean, 11 ± 5
  • NOTE: Mean percentage of CCR6-expressing CD8+ cells was significantly higher in TIL than in peripheral blood mononuclear cells from NSCLC patients (P = 0.000057) and healthy donors (P = 0.0000109). Statistical analyses were done using the two-tailed Student's t test.