Table 1.

Effect of γ-irradiation on Anxa1 expression in thymus stroma cells and thymocytes

γ-Irradiation treatmentExperimental groupCell typeAnxa1 normalized RNA expression (mean ± SD)Size (n)ANXA1 normalized protein expression (mean ± SD)Size (n)
NoneC57BL/6J (control)Thymus stroma cells3.53 ± 0.2231.20 ± 0.263
Thymocytes0.20 ± 0.080.43 ± 0.09
NRH1Thymus stroma cells3.64 ± 0.2631.40 ± 0.173
Thymocytes0.16 ± 0.080.50 ± 0.10
1 × 1.75 GyC57BL/6JThymus stroma cells7.43 ± 0.20 *32.30 ± 0.21 3
Thymocytes0.26 ± 0.070.44 ± 0.06
NRH1Thymus stroma cells9.39 ± 0.08 *, 33.50 ± 0.31 , §3
Thymocytes0.23 ± 0.070.50 ± 0.17
4 × 1.75 GyC57BL/6J (No TL)Thymus stroma cells3.48 ± 0.31101.06 ± 0.165
Thymocytes0.10 ± 0.060.34 ± 0.12
C57BL/6J (TL)Thymus stroma cells2.43 ± 0.29 *100.46 ± 0.14 5
Thymocytes0.11 ± 0.080.39 ± 0.17
NRH1 (No TL)Thymus stroma cells3.30 ± 0.33101.14 ± 0.195
Thymocytes0.10 ± 0.070.35 ± 0.14
NRH1 (TL)Thymus stroma cells2.22 ± 0.27 *100.49 ± 0.16 5
Thymocytes0.08 ± 0.040.32 ± 0.15
  • NOTE: Data are shown as the mean ± SD of normalized values obtained from three independent experiments. Statistically significant differences between the experimental groups were determined by a one-way ANOVA with a Bonferroni multiple comparison test.

    Abbreviations: No TL, irradiated mice that did not develop thymic lymphomas after completion of the latency period; TL, thymic lymphoma–bearing mice.

  • * P < 0.0001, compared with the corresponding value of the control stromal RNA expression.

  • P < 0.005, compared with the corresponding value of the control stromal protein expression.

  • P < 0.0001, compared with the corresponding value of the stromal RNA expression of 1.75 Gy–irradiated C57BL//6J mice.

  • § P < 0.003, compared with the corresponding value of the stromal protein expression of 1.75 Gy–irradiated C57BL//6J mice.