Table 1.

Description of colon cancer cases used for flow cytometry sorting of ALDH⁺ cells and the number of serial passages of xenograft lines

Case	Gender	Age	Location of tumor	Stage	No. passages *
CCXL 1	Female	52	Ascending	T3N0M0	7/8
CCXL 2	Female	70	Sigmoid	T3N1M0	7/9
CCXL 3	Male	79	Cecum	T3N1M0	9/10
CCXL 4 ^†	Male	75	Sigmoid	T2N0M0	12/12
CCXL 5	Male	41	Descending	T3N1M0	4/5
CCXL 6	Female	67	Ascending	T4N0M0	4/6
CCXL 7 ^†	Female	65	Ascending	T3N1M0	5/5

↵* Numerator gives the number of passages generated using Aldefluor⁺ cells and the denominator gives the total number of passages to date. All xenograft lines still continue to be passaged.
↵† In these two cases, xenograft tumors were first generated by implanting Aldefluor⁺ cells isolated directly from the primary tumor. In the other five cases, xenograft tumors were initially generated by implanting cells from the primary tumor in a NOD-SCID mouse and then subsequent xenografts were generated serially using Aldefluor⁺ cells isolated from a xenograft tumor.